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PROTOCOLS
T cell activation
Human and mouse T cell activation
CFSE labeling
Surface staining for flow cytometry
Molecular biology
Virus concentration
RNA extraction
Production of lentivirus
Recipes
Ammonium buffer for RBC lysis
Transfer buffer (WB)
Running buffer (WB)
RBC lysis buffer for DNA extraction
PEG
Ponceau
Work with mice
Animal room SOP
Cell isolation
Western blot
Bradford
Membrane staining with CTPS
Western blot